The present investigation was designed to check antimicrobial activity of combination of three extracts of Curcuma longa (CL), Ocimum santum (OS) and Gingiber officinale (GO) together and the development of suitable dosage form. Crude samples of CL, OS and GO were collected, air dried, powdered and extracted with 50% ethanol by maceration method and subjected for phytochemical analysis. Antimicrobial activity of individual extracts of CL, OS and GO CL, OS, GO and combined extract was carried out by the agar well diffusion method and tube dilution method. The extracts were tested on clinical isolates include aerobic, facultative bacteria namely Stapyhlococcus aureus ATCC 25921, Escherichia coli ATCC 25922, Bacillus subtilis and fungus Aspergillus niger. The combined extract showed greater antimicrobial activity than individual hydroalcoholic extracts. The minimum inhibitory concentration (MIC) of extracts varied from species of microorganisms, it was found to be 1 to 1.2 mg/ml. Further combined extract was used for preparation of topical formulations such as gel (1%) and ointment (1%). The further antimicrobial study of gel and ointment was compared which showed no loss of antimicrobial activity of extracts after six months. Then both the formulations were evaluated for physical characteristics and stability study.
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